Document Type
Article
Publication Date
12-1-2011
Abstract
It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. Mechanical stimulation was also combined with a Rho inhibitor to probe their coupled effects on FN. Mechanically stimulated cells revealed a localization of FN around the cell periphery as well as an increase in FN fibril formation. Mechanical stimulation coupled with chemical stimulation also revealed an increase in FN fibrils around the cell periphery. Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. These results show that mechanical stimulation directly affected FN reorganization and recruitment, despite perturbation by chemical stimulation. Our findings will help elucidate the mechanisms of FN biosynthesis and organization by furthering the link of the role of mechanics with FN.
DOI
10.1038/srep00147
Published Article/Book Citation
Steward, R., Cheng, CM., Ye, J. et al. Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts. Sci Rep 1, 147 (2011). https://doi.org/10.1038/srep00147
Repository Citation
Steward, Robert L.; Cheng, Chao Min; Ye, Jonathan D.; Bellin, Robert M.; and Leduc, Philip R., "Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts" (2011). Biology Department Faculty Scholarship. 2.
https://crossworks.holycross.edu/bio_fac_scholarship/2